In vitro growth of calcium pyrophosphate crystals in polyacrylamide gels
نویسندگان
چکیده
منابع مشابه
Survival of calcium pyrophosphate crystals in stored synovial fluids.
Eleven synovial fluids containing calcium pyrophosphate dihydrate (CPPD) were examined repeatedly over an eight week period to assess whether storage conditions and duration influenced the number of crystals present. Aliquots of each fluid were stored at room temperature, 4 degrees C, and -70 degrees C. At -70 degrees C there was no change in crystal count after eight weeks' storage. At room te...
متن کاملDissolution of calcium pyrophosphate crystals by polyphosphates: an in vitro and ex vivo study.
OBJECTIVE To determine the dissolving ability (DA) of linear pentasodium tripolyphosphate (PSTP), cyclic trisodium metaphosphate (TSMP), polymeric sodium metaphosphate (SMP) on synthetic crystals of calcium pyrophosphate dihydrate (CPPD) and on crystalline aggregates of menisci from patients with chondrocalcinosis (CC). METHODS Synthetic CPPD crystals were mixed with phosphate buffered saline...
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Although osteoarthritis (OA) is defined as a cartilage disease, synovitis involving mononuclear cell infiltration and overexpression of proinflammatory mediators is common in early and late OA. Calcium crystals deposition is thought to be a factor that likely contributes to synovial inflammation. In recent years, significant interest has emerged in the beneficial health effects attributed to th...
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Mujer de 61 años de edad, que acudió a consulta por dolor moderado y limitación funcional de la rodilla izquierda de 4 meses de evolución. La paciente tiene historia de esclerosis sistémica de 2 años de evolución. Al examen físico la rodilla tenía aumento de volumen e hipertermia local. La velocidad de sedimentación globular fue de 47 mm/h. Las radiografías de las rodillas fueron normales. La u...
متن کاملDemonstration of rhodanese activity in polyacrylamide gels.
Rhodanese activity from crude extracts of Thiobacillus sp. strain IV-85 was demonstrated in polyacrylamide gels after incubation in the reaction mixture by staining with dichloroindophenol in the presence of methylphenazonium methosulfate. The sensitivity of the staining system was found to be 8 x 10 moles of sulfite.
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ژورنال
عنوان ژورنال: Annals of the Rheumatic Diseases
سال: 1983
ISSN: 0003-4967
DOI: 10.1136/ard.42.suppl_1.100